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KMID : 1005220090240010021
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Journal of Embryo Transfer
2009 Volume.24 No. 1 p.21 ~ p.27
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Effects of the Addition of and BSA on the Development of Porcine Embryos
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Park Yong-Soo
Kim Myung-Sin
Park Hum-Dae
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Abstract
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This study was performed to elucidate the effects of addition of and bovine serum albumin (BSA) in vitro maturation (IVM) and in vitro culture (IVC) medium on porcine embryo production. The development rate to the 2 cell () and blastocyst stages () with different BSA concentrations in IVM medium were similar among treatment groups. Blastocyst hatching rate was significantly higher in the control group (0.0mg/ml) than in the group of 1.0mg/ml supplement (20.0% vs. 0.0%; p<0.05). The development rate to the 2 cell () and blastocyst stages () with different concentrations in IVM medium was similar among treatment groups. The development rate to the blastocyst was significantly higher in the group of 1.0mg/ml(15.3%) than in the group of 0.5mg/ml supplement (7.6%, p<0.05). The development rate to the 2 cell and blastocyst stages following the first addition of in IVM medium was significantly higher in the control group (77.0% and 18.9%) and was (77.2% and 16.9%) greater than that observed in other treatment groups (p<0.05). The development rate to the 2 cell stage () and blastocyst stages () with different BSA concentrations in IVC medium was similar among treatment groups. However, blastocyst hatching rate was significantly higher in the group of 3.0mg/ml supplement (30.0%) than in the control group (0.0%; p<0.05). The development rate to the 2 cell stage (), blastocyst () and hatching stages () were not different. The development rate to the 2 cell stage (), blastocyst () and hatching stages () at the different culture periods were similar among treatment groups. This study suggested that if the addition level and periods of addition are adjusted, it is possible to replace BSA in the in vitro porcine embryo production.
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KEYWORD
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porcine, BSA, , in vitro culture
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